ABNORMAL BEHAVIOR OF SPINDLE DURING MICROSPOROGENESIS OF Passiflora (PASSIFLORACEAE)

Paulo Roberto Peres Kiihl, Maria Francieli Barragan, Sheila Pires dos Santos, Sara Mataroli de Godoy, Andréia Rodrigues Alonso-Pereira, Neusa Maria Colauto Stenzel, Claudicéia Risso-Pascotto

Resumo


Passiflora genus, Passifloraceae family, has more than 500 species and 120 of them are native species of Brazil. All species produce fruits that are used as food, medicine and decoration. Floral buttons of five species were collected and fixed in a mixture of ethanol and acetic acid (3:1). The slides were prepared by squashing and staining with 1% propionic carmine. Results showed that during microsporogenesis there were few irregularities, mostly frequently related to chromosome irregular segregation as: precocious migration to poles in metaphase I and II, non-oriented bivalent chromosomes at metaphase I and II, and laggard chromosomes in anaphase I and II, forming micronuclei in telophases I and II and tetrad with microcyte. Another observed irregularity is related to the organization of spindle fibers in meiosis II as they organize themselves in T and V shapes and in sequential spindle. However, in the V-shaped spindle configuration, there was fusion between two nuclei that were close, forming triads instead of tetrads. Irregular chromosome segregation, abnormal spindles and irregularities in the cytokinesis process were responsible for the formation of monads, dyads, triads and polyads. However, the pollen grain viability was not harmed, presenting an 83.98% to 98.59% fertility variation.

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DOI: https://doi.org/10.25110/arqsaude.v14i3.2010.3666